Please use this identifier to cite or link to this item: doi:10.22028/D291-29495
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Title: A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineered Corynebacterium glutamicum
Author(s): Rohles, Christina Maria
Gläser, Lars
Kohlstedt, Michael
Gießelmann, Gideon
Pearson, Samuel
del Campo Bécares, Aránzazu
Becker, Judith
Wittmann, Christoph
Language: English
Title: Green chemistry : GC
Volume: 20
Issue: 20
Startpage: 4662
Endpage: 4674
Publisher/Platform: RSC
Year of Publication: 2018
Publikation type: Journal Article
Abstract: In the present work, we established the bio-based production of glutarate, a carbon-5 dicarboxylic acid with recognized value for commercial plastics and other applications, using metabolically engineered Corynebacterium glutamicum. The mutant C. glutamicum AVA-2 served as a starting point for strain development, because it secreted small amounts of glutarate as a consequence of its engineered 5-aminovalerate pathway. Starting from AVA-2, we overexpressed 5-aminovalerate transaminase (gabT) and glutarate semialdehyde dehydrogenase (gabD) under the control of the constitutive tuf promoter to convert 5-aminovalerate further to glutarate. The created strain GTA-1 formed glutarate as a major product, but still secreted 5-aminovalerate as well. This bottleneck was tackled at the level of 5-aminovalerate re-import. The advanced strain GTA-4 overexpressed the newly discovered 5-aminovalerate importer NCgl0464 and formed glutarate from glucose in a yield of 0.27 mol mol−1. In a fed-batch process, GTA-4 produced more than 90 g L−1 glutarate from glucose and molasses based sugars in a yield of up to 0.70 mol mol−1 and a maximum productivity of 1.8 g L−1 h−1, while 5-aminovalerate was no longer secreted. The bio-based glutaric acid was purified to >99.9% purity. Interfacial polymerization and melt polymerization with hexamethylenediamine yielded bionylon-6,5, a polyamide with a unique structure.
DOI of the first publication: 10.1039/C8GC01901K
URL of the first publication: https://pubs.rsc.org/en/content/articlelanding/2018/gc/c8gc01901k#!divAbstract
Link to this record: hdl:20.500.11880/28209
http://dx.doi.org/10.22028/D291-29495
ISSN: 1463-9270
1463-9262
Date of registration: 25-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:UniBib – Die Universitätsbibliographie

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