Please use this identifier to cite or link to this item: doi:10.22028/D291-29413
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Title: Bifunctional Hydrogels Containing the Laminin Motif IKVAV Promote Neurogenesis
Author(s): Farrukh, Aleeza
Ortega, Felipe
Fan, Wenqiang
Marichal, Nicolás
Paez, Julieta I.
Berninger, Benedikt
del Campo Bécares, Aránzazu
Salierno, Marcelo J.
Language: English
Title: Stem cell reports
Volume: 9
Issue: 5
Startpage: 1432
Endpage: 1440
Publisher/Platform: Elsevier
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing β1-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties.
DOI of the first publication: 10.1016/j.stemcr.2017.09.002
URL of the first publication: https://www.sciencedirect.com/science/article/pii/S2213671117303855
Link to this record: hdl:20.500.11880/28194
http://dx.doi.org/10.22028/D291-29413
ISSN: 2213-6711
Date of registration: 25-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Chemie
Professorship: NT - Prof. Dr. Aránzazu del Campo
Collections:UniBib – Die Universitätsbibliographie

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