Please use this identifier to cite or link to this item: doi:10.22028/D291-29462
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Title: Thin Film Composite Silicon Elastomers for Cell Culture and Skin Applications : Manufacturing and Characterization
Author(s): Boyadzhieva, Silviya
Fischer, Sarah C.
Lösch, Svenja
Rutz, Angela
Arzt, Eduard
Kruttwig, Klaus
Language: English
Title: Journal of Visualized Experiments : JoVE
Issue: 137
Startpage: 1
Endpage: 16
Publisher/Platform: JoVE
Year of Publication: 2018
Publikation type: Journal Article
Abstract: In this protocol, we present methods to fabricate thin elastomer composite films for advanced cell culture applications and for the development of skin adhesives. Two different poly-(dimethyl siloxanes) (PDMS and soft skin adhesive (SSA)), have been used for in depth investigation of biological effects and adhesive characteristics. The composite films consist of a flexible backing layer and an adhesive top coating. Both layers have been manufactured by doctor blade application technique. In the present investigation, the adhesive behavior of the composite films has been investigated as a function of the layer thickness or a variation of the Young's modulus of the top layer. The Young's modulus of PDMS has been changed by varying the base to crosslinker mixing ratio. In addition, the thickness of SSA films has been varied from approx. 16 µm to approx. 320 µm. Scanning electron microscopy (SEM) and optical microscopy have been used for thickness measurements. The adhesive properties of elastomer films depend strongly on the film thickness, the Young's modulus of the polymers and surface characteristics. Therefore, normal adhesion of these films on glass substrates exhibiting smooth and rough surfaces has been investigated. Pull-off stress and work of separation are dependent on the mixing ratio of silicone elastomers. Additionally, the thickness of the soft skin adhesive placed on top of a supportive backing layer has been varied in order to produce patches for skin applications. Cytotoxicity, proliferation and cellular adhesion of L929 murine fibroblasts on PDMS films (mixing ratio 10:1) and SSA films (mixing ratio 50:50) have been conducted. We have shown here, for the first time, the side by side comparison of thin composite films manufactured of both polymers and present the investigation of their biological- and adhesive properties.
DOI of the first publication: 10.3791/57573
Link to this record: hdl:20.500.11880/28154
http://dx.doi.org/10.22028/D291-29462
ISSN: 1940-087X
Date of registration: 21-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Materialwissenschaft und Werkstofftechnik
Professorship: NT - Prof. Dr. Eduard Arzt
Collections:UniBib – Die Universitätsbibliographie

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