Please use this identifier to cite or link to this item: doi:10.22028/D291-29274
Volltext verfügbar? / Dokumentlieferung
Title: Single-molecule force spectroscopy of fast reversible bonds
Author(s): Blass, Johanna
Albrecht, Marcel
Wenz, Gerhard
Zang, Yan Nan
Bennewitz, Roland
Language: English
Title: Physical Chemistry Chemical Physics
Volume: 19
Issue: 7
Startpage: 5239
Endpage: 5245
Publisher/Platform: Royal Society of Chemistry (RSC)
Year of Publication: 2017
Publikation type: Journal Article
Abstract: In single-molecule force spectroscopy, the unbinding force is often used to quantify the interaction strength of single molecular bonds. We analyze force spectroscopy of fast reversible bonds probed in thermodynamic equilibrium by considering the dynamics of force probe and molecular linker. The effect of cantilever and linker dynamics is systematically addressed by measuring the unbinding force of single cyclodextrin inclusion complexes by atomic force spectroscopy for a variety of molecular linkers and varying force probe stiffness. The unbinding force of individual bonds probed in thermodynamic equilibrium is not unique for the molecular system but scales with Image ID:c6cp07532k-t1.gif, the square root of the force probe stiffness, and is largely independent of the molecular linker stiffness. The observations are explained by an effective potential resulting from fast linker fluctuations and fast rebinding kinetics which is probed by an AFM cantilever. The slow cantilever dynamics in the kHz range act as mechanical low pass filter, allowing for fast rebinding kinetics of the molecular complex in the order of 106 kHz. The binding energy of the complex can be estimated from the unbinding force as a function of cantilever stiffness, however with some uncertainty arising from lack of a model in three dimensions.
DOI of the first publication: 10.1039/C6CP07532K
Link to this record: hdl:20.500.11880/28133
ISSN: 1463-9076
Date of registration: 17-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Keiner Professur zugeordnet
Collections:UniBib – Die Universitätsbibliographie

Files for this record:
There are no files associated with this item.

Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.