Please use this identifier to cite or link to this item: doi:10.22028/D291-29271
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Title: Oil droplet formation on pellicle covered tooth surfaces studied with environmental scanning electron microscopy
Author(s): Peckys, Diana B.
de Jonge, Niels
Hannig, Matthias
Language: English
Title: Journal of Microscopy
Volume: 274
Issue: 3
Startpage: 158
Endpage: 167
Publisher/Platform: Wiley-Blackwell - STM
Year of Publication: 2019
Publikation type: Journal Article
Abstract: Lipophilic components are known to modulate the process of bioadhesion on the tooth surface. However, the presence of lipid droplets at the acquired pellicle under oral conditions has not been demonstrated, yet. The purpose of the present study was to establish a method for direct visualisation of lipids on the surface of hydrated, pellicle covered tooth samples by environmental scanning electron microscopy (ESEM), and to use this technique for studying the effects of rinsing with edible oils on the acquired pellicle under in vivo conditions. In situ pellicle formation was performed by 3 min exposure of enamel and dentin specimens in the oral cavity of volunteers. Subsequently, the volunteers rinsed in vivo with safflower oil or linseed oil for 30 s, and the specimens were further carried intraorally for periods from 0 min up to several hours. After intraoral exposure the specimens were treated by osmium tetroxide vapour, and were subsequently analysed by ESEM. This technique was capable to directly visualise the presence of lipid droplets at the pellicle's surface under hydrated conditions. ESEM analyses revealed that surface bound nano- and micro-sized lipid droplets were present at the acquired pellicle's surface even several hours after rinsing with edible oils indicating that these droplets had tightly adhered to the pellicle surface. Pellicle modification by edible oil rinsing as demonstrated in the present study might have the potential to be beneficial as an adjunct in dental prophylaxis.
DOI of the first publication: 10.1111/jmi.12794
Link to this record: hdl:20.500.11880/28080
ISSN: 0022-2720
Date of registration: 14-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
M - Medizinische Fakultät
Department: NT - Physik
M - Zahn-, Mund- und Kieferheilkunde
Professorship: NT - Keiner Professur zugeordnet
M - Prof. Dr. Matthias Hannig
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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