Please use this identifier to cite or link to this item: doi:10.22028/D291-29241
Volltext verfügbar? / Dokumentlieferung
Title: The 2018 correlative microscopy techniques roadmap
Author(s): Ando, Toshio
Bhamidimarri, Satya Prathyusha
Brending, Niklas
Colin-York, H.
Collinson, Lucy
de Jonge, Niels
de Pablo, P. J.
Debroye, Elke
Eggeling, Christian
Franck, Christian
Fritzsche, Marco
Gerritsen, Hans
Giepmans, Ben N. G.
Grunewald, Kay
Hofkens, Johan
Hoogenboom, Jacob P.
Janssen, Kris P. F.
Kaufman, Rainer
Klumpermann, Judith
Kurniawan, Nyoman
Kusch, Jana
Liv, Nalan
Parekh, Viha
Peckys, Diana B.
Rehfeldt, Florian
Reutens, David C.
Roeffaers, Maarten B. J.
Salditt, Tim
Schaap, Iwan A. T.
Schwarz, Ulrich S.
Verkade, Paul
Vogel, Michael W.
Wagner, Richard
Winterhalter, Mathias
Yuan, Haifeng
Zifarelli, Giovanni
Language: English
Title: Journal of Physics D : Applied Physics
Volume: 51
Issue: 44
Startpage: 443001
Publisher/Platform: IOP Publishing
Year of Publication: 2018
Publikation type: Journal Article
Abstract: Developments in microscopy have been instrumental to progress in the life sciences, and many new techniques have been introduced and led to new discoveries throughout the last century. A wide and diverse range of methodologies is now available, including electron microscopy, atomic force microscopy, magnetic resonance imaging, small-angle x-ray scattering and multiple super-resolution fluorescence techniques, and each of these methods provides valuable read-outs to meet the demands set by the samples under study. Yet, the investigation of cell development requires a multi-parametric approach to address both the structure and spatio-temporal organization of organelles, and also the transduction of chemical signals and forces involved in cell-cell interactions. Although the microscopy technologies for observing each of these characteristics are well developed, none of them can offer read-out of all characteristics simultaneously, which limits the information content of a measurement. For example, while electron microscopy is able to disclose the structural layout of cells and the macromolecular arrangement of proteins, it cannot directly follow dynamics in living cells. The latter can be achieved with fluorescence microscopy which, however, requires labelling and lacks spatial resolution. A remedy is to combine and correlate different readouts from the same specimen, which opens new avenues to understand structure-function relations in biomedical research. At the same time, such correlative approaches pose new challenges concerning sample preparation, instrument stability, region of interest retrieval, and data analysis. Because the field of correlative microscopy is relatively young, the capabilities of the various approaches have yet to be fully explored, and uncertainties remain when considering the best choice of strategy and workflow for the correlative experiment. With this in mind, the Journal of Physics D: Applied Physics presents a special roadmap on the correlative microscopy techniques, giving a comprehensive overview from various leading scientists in this field, via a collection of multiple short viewpoints.
DOI of the first publication: 10.1088/1361-6463/aad055
Link to this record: hdl:20.500.11880/28064
ISSN: 0022-3727
Date of registration: 14-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Keiner Professur zugeordnet
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

Files for this record:
There are no files associated with this item.

Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.