Please use this identifier to cite or link to this item: doi:10.22028/D291-29239
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Title: Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages
Author(s): Vanhecke, Dimitri
Kuhn, Dagmar A.
Jimenez de Aberasturi, Dorleta
Balog, Sandor
Milosevic, Ana
Urban, Dominic
Peckys, Diana
de Jonge, Niels
Parak, Wolfgang J.
Petri-Fink, Alke
Rothen-Rutishauser, Barbara
Language: English
Title: Beilstein Journal of Nanotechnology
Volume: 8
Startpage: 2396
Endpage: 2409
Publisher/Platform: Beilstein-Institut
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Little is known about the simultaneous uptake of different engineered nanoparticle types, as it can be expected in our daily life. In order to test such co-exposure effects, murine macrophages (J774A.1 cell line) were incubated with gold (AuNPs) and iron oxide nanoparticles (FeO x NPs) either alone or combined. Environmental scanning electron microscopy revealed that single NPs of both types bound within minutes on the cell surface but with a distinctive difference between FeO x NPs and AuNPs. Uptake analysis studies based on laser scanning microscopy, transmission electron microscopy, and inductively coupled plasma optical emission spectrometry revealed intracellular appearance of both NP types in all exposure scenarios and a time-dependent increase. This increase was higher for both AuNPs and FeO x NPs during co-exposure. Cells treated with endocytotic inhibitors recovered after co-exposure, which additionally hinted that two uptake mechanisms are involved. Cross-talk between uptake pathways is relevant for toxicological studies: Co-exposure acts as an uptake accelerant. If the goal is to maximize the cellular uptake, e.g., for the delivery of pharmaceutical agents, this can be beneficial. However, co-exposure should also be taken into account in the case of risk assessment of occupational settings. The demonstration of co-exposure-invoked pathway interactions reveals that synergetic nanoparticle effects, either positive or negative, must be considered for nanotechnology and nanomedicine in particular to develop to its full potential.
DOI of the first publication: 10.3762/bjnano.8.239
Link to this record: hdl:20.500.11880/28063
ISSN: 2190-4286
Date of registration: 14-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Keiner Professur zugeordnet
Collections:UniBib – Die Universitätsbibliographie

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