Please use this identifier to cite or link to this item: doi:10.22028/D291-29236
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Title: Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy
Author(s): Hermannsdörfer, Justus
de Jonge, Niels
Language: English
Title: Journal of Visualized Experiments : JoVE
Issue: 120
Publisher/Platform: JoVE
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Samples fully embedded in liquid can be studied at a nanoscale spatial resolution with Scanning Transmission Electron Microscopy (STEM) using a microfluidic chamber assembled in the specimen holder for Transmission Electron Microscopy (TEM) and STEM. The microfluidic system consists of two silicon microchips supporting thin Silicon Nitride (SiN) membrane windows. This article describes the basic steps of sample loading and data acquisition. Most important of all is to ensure that the liquid compartment is correctly assembled, thus providing a thin liquid layer and a vacuum seal. This protocol also includes a number of tests necessary to perform during sample loading in order to ensure correct assembly. Once the sample is loaded in the electron microscope, the liquid thickness needs to be measured. Incorrect assembly may result in a too-thick liquid, while a too-thin liquid may indicate the absence of liquid, such as when a bubble is formed. Finally, the protocol explains how images are taken and how dynamic processes can be studied. A sample containing AuNPs is imaged both in pure water and in saline.
DOI of the first publication: 10.3791/54943
Link to this record: hdl:20.500.11880/28053
ISSN: 1940-087X
Date of registration: 10-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Keiner Professur zugeordnet
Collections:UniBib – Die Universitätsbibliographie

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