Please use this identifier to cite or link to this item: doi:10.22028/D291-29601
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Title: Mucus-penetrating solid lipid nanoparticles for the treatment of cystic fibrosis: Proof of concept, challenges and pitfalls
Author(s): Nafee, N.
Forier, Katrien
Braeckmans, Kevin
Schneider, Marc
Language: English
Title: European journal of pharmaceutics and biopharmaceutics : EJPB
Volume: 124
Startpage: 125
Endpage: 137
Publisher/Platform: Elsevier
Year of Publication: 2018
Publikation type: Journal Article
Abstract: Nanocarrier-mediated transmucosal drug delivery based on conventional mucoadhesive, muco-inert or mucus-penetrating nanoparticles (NPs) is a growing field especially in challenging diseases like cystic fibrosis (CF). Efficacy of such systems dictates profound investigation of particle-mucus interaction and factors governing the whole process. Although variable techniques studying particle diffusion in mucus have been introduced, standardized procedures are lacking. The study comprised different methods based on micro- and macro-displacement as well as colloidal stability and turbidimetric experiments. Artificial sputum medium (ASM), CF sputum and mucus-secreting cell line (Calu-3 air interface culture, AIC) were applied. Solid lipid nanoparticles (SLNs) coated with variable hydrophilic sheath (poloxamer, Tween 80 or PVA) represented the nanocarriers under investigation. Both micro-displacement studies based on single particle tracking and macro-displacement experiments based on 3D-time laps confocal imaging revealed faster diffusion of poloxamer- > Tween- > PVA-coated SLNs. Compared to ASM, CF sputum showed not only lower diffusion rates but also remarkable discrepancies in particle-mucus diffusion rate due to sputum heterogenicity. Meanwhile, in case of Calu-3 AIC, thickness of the mucosal layer as well as density of mucus network were key determinants in the diffusion process. The points emphasized in this study highlight the road towards in vivo relevant particle-mucus interaction research.
DOI of the first publication: 10.1016/j.ejpb.2017.12.017
URL of the first publication:
Link to this record: hdl:20.500.11880/27986
ISSN: 1873-3441
Date of registration: 1-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Marc Schneider
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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