Please use this identifier to cite or link to this item: doi:10.22028/D291-29600
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Title: Silica nanoparticles of microrods enter lung epithelial cells
Author(s): Tschernig, Thomas
Fischer, Thorben
Grissmer, Alexander
Beckmann, Anja
Meier, Carola
Lipp, Peter
Schneider, Marc
Language: English
Title: Biomedical reports : an international journal devoted to biomedical research
Volume: 9
Issue: 2
Startpage: 156
Endpage: 160
Publisher/Platform: Spandidos Publications
Year of Publication: 2018
Publikation type: Journal Article
Abstract: A novel type of microparticle has recently been engineered. It consists of amorphous silica nanoparticles and has a corncob-like shape. It has already been demonstrated in vivo that alveolar macrophages in the lung are able to engulf this particulate carrier and that it also functions successfully as a gene delivery system. This subsequently raises the question as to whether epithelial cells may also be possible targets for these microrods. For this purpose, the alveolar epithelial cell line A549 was used presently. The epithelial character of these confluent cells was documented by the presence of tight junctions using a freeze-fracture technique and transmission electron microscopy. A toxic effect of the particles incubated with these cells was largely excluded. The interaction of the microparticles with the epithelial cells was observed using confocal microscopy and live cell imaging. Interestingly, the particles entered the epithelial cells within hours. After 1 day, the intracellular particles began to disaggregate and release the silica nanoparticles. Thus, even epithelial cells may serve as targets for this novel carrier and gene delivery system. This is particularly important since safe and effective gene delivery remains an unsolved problem. In addition, delivery of anti-cancer and anti-infective drugs may be an application of this novel particulate carrier.
DOI of the first publication: 10.3892/br.2018.1117
URL of the first publication: https://www.spandidos-publications.com/br/9/2/156/abstract
Link to this record: hdl:20.500.11880/27985
http://dx.doi.org/10.22028/D291-29600
ISSN: 2049-9434
Date of registration: 1-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Marc Schneider
Collections:UniBib – Die Universitätsbibliographie

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