Please use this identifier to cite or link to this item: doi:10.22028/D291-29590
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Title: Design and Characterization of Surface‐Crosslinked Gelatin Nanoparticles for the Delivery of Hydrophilic Macromolecular Drugs
Author(s): Baseer, Abdul
Koenneke, Aljoscha
Zapp, Josef
Khan, Saeed A.
Schneider, Marc
Language: English
Title: Macromolecular chemistry and physics : MCP
Volume: 220
Issue: 18
Startpage: 1
Endpage: 12
Publisher/Platform: Wiley-VCH
Year of Publication: 2019
Publikation type: Journal Article
Abstract: For nanotechnology enabled delivery of hydrophilic protein‐based drugs, several polymer‐based carrier systems have been used in the past to protect the sensitive load and to facilitate cellular uptake and crossing of biological barriers. This study uses gelatin, a natural and biodegradable macromolecule, as carrier material which is approved for several applications. Nanoprecipitation is used to form nanoparticles and to maintain the physicochemical integrity of gelatin, hydrophilic crosslinkers, e.g., paraformaldehyde, glutaraldehyde, carbodiimide, and transglutaminase are employed. However, these crosslinkers diffuse homogenously into the carrier matrix also crosslinking the polymeric matrix with the entrapped protein‐based molecules thus rendering it inactive. Hence a hydrophobic zero‐length crosslinker, diisopropylcarbodiimide, is applied to avoid diffusion into the particles. This will provide an opportunity to encapsulate protein‐based drugs in the non‐crosslinked matrix. The hypothesis of surface crosslinking is proven by the extent of crosslinking and more importantly by encapsulation and the release of lysozyme as a model hydrophilic protein. Furthermore, essential process parameters are evaluated such as crosslinker concentration, crosslinking time and crosslinking reaction temperature with regard to the effect on particle size, size distribution and zeta‐potential of gelatin nanoparticles. The optimum formulation results in the production of gelatin nanoparticles with 200‐300 nm and a polydispersity index < 0.2.
DOI of the first publication: 10.1002/macp.201900260
URL of the first publication: https://onlinelibrary.wiley.com/doi/abs/10.1002/macp.201900260
Link to this record: hdl:20.500.11880/27976
http://dx.doi.org/10.22028/D291-29590
ISSN: 1521-3935
1616-5187
1022-1352
Date of registration: 1-Oct-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Marc Schneider
Collections:UniBib – Die Universitätsbibliographie

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