Please use this identifier to cite or link to this item: doi:10.22028/D291-28835
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Title: High-resolution, label-free two-photon imaging of diseased human corneas.
Author(s): Goncalves Batista, Ana Maria
Breunig, Hans Georg
König, Aisada
Schindele, Andreas
Hager, Tobias
Seitz, Berthold
König, Karsten
Language: English
Title: Journal of Biomedical Optics
Volume: 23
Issue: 3
Startpage: 1
Endpage: 8
Publisher/Platform: SPIE
Year of Publication: 2018
Publikation type: Journal Article
Abstract: The diagnosis of corneal diseases may be improved by monitoring the metabolism of cells and the structural organization of the stroma using two-photon imaging (TPI). We used TPI to assess the differences between nonpathological (NP) human corneas and corneas diagnosed with either keratoconus, Acanthamoeba keratitis, or stromal corneal scars. Images were acquired using a custom-built five-dimensional laser-scanning microscope with a broadband sub-15 femtosecond near-infrared pulsed excitation laser and a 16-channel photomultiplier tube detector in combination with a time-correlated single photon counting module. Morphological alterations of epithelial cells were observed for all pathologies. Moreover, diseased corneas showed alterations to the cells' metabolism that were revealed using the NAD(P)H free to protein-bound ratios. The mean autofluorescence lifetime of the stroma and the organization of the collagen fibers were also significantly altered due to the pathologies. We demonstrate that TPI can be used to distinguish between NP and diseased human corneas, based not only on alterations of the cells' morphology, which can also be evaluated using current clinical devices, but on additional morphological and functional features such as the organization of the stroma and the cells' metabolism. Therefore, TPI could become an efficient tool for diagnosing corneal diseases and better understanding the biological processes of the diseases.
DOI of the first publication: 10.1117/1.JBO.23.3.036002
URL of the first publication:
Link to this record: hdl:20.500.11880/27955
ISSN: 1560-2281
Date of registration: 30-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Systems Engineering
Professorship: NT - Prof. Dr. Karsten König
Collections:Die Universitätsbibliographie

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