Please use this identifier to cite or link to this item: doi:10.22028/D291-29540
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Title: Impact of cigarette-smoking on sperm DNA methylation and its effect on sperm parameters
Author(s): Alkhaled, Yasar
Laqqan, Mohammed
Tierling, Sascha
Lo Porto, Christina
Amor, Houda
Hammadeh, M. E.
Language: English
Title: Andrologia
Volume: 50
Issue: 4
Publisher/Platform: Wiley-Blackwell - STM
Year of Publication: 2018
Publikation type: Journal Article
Abstract: DNA methylation is an epigenetic modification of the genome. The purpose of this study was to determine the influence of cigarette-smoking on sperm DNA methylation from a genomewide survey of sperm samples and to ascertain its effect on sperm parameters. Twenty-eight sperm DNA samples (from 14 fertile smokers as a case study and 14 proven fertile nonsmokers as controls) were subjected to Infinium 450K BeadChip arrays to identify the changes in the DNA methylation level between the two groups. Then, deep bisulphite sequencing was used to validate five CpGs on 78 samples. The results from the Infinium 450K found that only 11 CpGs showed a significant difference in DNA methylation between the case and the control groups. Five CpGs of the eleven (cg00648582, cg0932376, cg19169023, cg23841288 and cg27391564) underwent deep bisulphite sequencing where cg00648582, related to the PGAM5 gene, and the cg23841288 CpGs, related to the PTPRN2 gene amplicons, showed a significant increase in their DNA methylation level in more than one CpG in the case group. In contrast, a significant decrease was found at cg19169023 and at its various neighbouring CpGs in the TYRO3 gene-related amplicons. Furthermore, this study demonstrated a significant correlation between the variation in sperm DNA methylation level and standard sperm parameters in the case group.
DOI of the first publication: 10.1111/and.12950
Link to this record: hdl:20.500.11880/27946
ISSN: 1439-0271
Date of registration: 30-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Jörn Walter
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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