Please use this identifier to cite or link to this item:Volltext verfügbar? / Dokumentlieferung
|Title:||Comparison of initial oral microbiomes of young adults with and without cavitated dentin caries lesions using an in situ biofilm model|
Laczny, Cedric C.
Lo Porto, Christina
Walter, Jörn Erik
|Year of Publication:||2018|
|Publikation type:||Journal Article|
|Abstract:||Dental caries is caused by acids released from bacterial biofilms. However, the in vivo formation of initial biofilms in relation to caries remains largely unexplored. The aim of this study was to compare the oral microbiome during the initial phase of bacterial colonization for individuals with (CC) and without (NC) cavitated dentin caries lesions. Bovine enamel slabs on acrylic splints were worn by the volunteers (CC: 14, NC: 13) for in situ biofilm formation (2 h, 4 h, 8 h, 1 ml saliva as reference). Sequencing of the V1/V2 regions of the 16S rRNA gene was performed (MiSeq). The relative abundances of individual operational taxonomic units (OTUs) were compared between samples from the CC group and the NC group. Random forests models were furthermore trained to separate the groups. While the overall heterogeneity did not differ substantially between CC and NC individuals, several individual OTUs were found to have significantly different relative abundances. For the 8 h samples, most of the significant OTUs showed higher relative abundances in the CC group, while the majority of significant OTUs in the saliva samples were more abundant in the NC group. Furthermore, using OTU signatures enabled a separation between both groups, with area-under-the-curve (AUC) values of ~0.8. In summary, the results suggest that initial oral biofilms provide the potential to differentiate between CC and NC individuals.|
|DOI of the first publication:||10.1038/s41598-018-32361-x|
|Link to this record:||hdl:20.500.11880/27910|
|Date of registration:||26-Sep-2019|
|Faculty:||NT - Naturwissenschaftlich- Technische Fakultät|
|Department:||NT - Biowissenschaften|
|Professorship:||NT - Prof. Dr. Jörn Walter|
|Collections:||UniBib – Die Universitätsbibliographie|
Files for this record:
There are no files associated with this item.
Items in SciDok are protected by copyright, with all rights reserved, unless otherwise indicated.