Please use this identifier to cite or link to this item: doi:10.22028/D291-29436
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Title: Cigarette smoking induces only marginal changes in sperm DNA methylation levels of patients undergoing intracytoplasmic sperm injection treatment
Author(s): Alkhaled, Yaser
Tierling, Sascha
Laqqan, Mohammed
Lo Porto, Christina
Hammadeh, M. E.
Language: English
Title: Andrologia
Volume: 50
Issue: 1
Publisher/Platform: Wiley-Blackwell - STM
Year of Publication: 2017
Publikation type: Journal Article
Abstract: DNA methylation plays important roles in genome stability and regulation of gene expression. This study was designed to determine the influence of cigarette smoking on sperm DNA methylation. From a genome-wide survey on sperm samples, differentially methylated target CpGs should be selected and subjected to local deep bisulphite sequencing. Obtained methylation data are compared to sperm parameters and (ICSI) outcome. Similar to pilot study, samples were subjected to Infinium 450K BeadChip arrays to identify alterations in sperm DNA methylation between smokers and nonsmokers males. Routine testing on a significantly altered CpG site was performed on more samples using local deep bisulphite sequencing. Of approximately 485,000 CpG sites analysed, only seven CpGs were found to show a significant DNA methylation difference of >20% with the top six CpGs overlapping common SNP sites. The remaining CpG site (cg19455396) is located in intron 12 of the TAP2 gene. The results of deep bisulphite sequencing showed only a tendency towards hypomethylation in the smoking group. This study could not detect biologically relevant CpG positions that are altered in sperm DNA methylation on the influence of cigarette smoking beyond individual-specific effects that may be caused by other environmental factors.
DOI of the first publication: 10.1111/and.12818
Link to this record: hdl:20.500.11880/27892
http://dx.doi.org/10.22028/D291-29436
ISSN: 1439-0271
1439-0272
0303-4569
Date of registration: 25-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Jörn Walter
Collections:UniBib – Die Universitätsbibliographie

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