Please use this identifier to cite or link to this item: doi:10.22028/D291-29433
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Title: Aberrant DNA methylation patterns of human spermatozoa in current smoker males
Author(s): Laqqan, Mohammed
Tierling, Sascha
Alkhaled, Yaser
Lo Porto, Christina
Solomayer, E. F.
Hammadeh, M. E.
Language: English
Title: Reproductive toxicology
Volume: 71
Startpage: 126
Endpage: 133
Publisher/Platform: Elsevier
Year of Publication: 2017
Publikation type: Journal Article
Abstract: The purpose of this study was to investigate the impact of current cigarette smoking on sperm DNA methylation patterns. A total of 108 males (51 current smokers and 57 never smoked males) were included in the study. Using 450 BeadChip Arrays, the differentially methylated CpGs between current smokers (n=15) and never smoked males (n=15) were identified. Out of significantly 11 CpGs identified, 2 CpGs namely cg07869343 and cg19169023, which are located in the MAPK8IP3 and TKR genes were selected for further analysis. Using deep bisulfite sequencing in an independent cohort of current smokers (n=36) and never smoked males (n=42), 6 and 1 CpGs showed a significant difference in the MAPK8IP (CpG3, CpG5, CpG6, CpG7, CpG8, and CpG21) and in the TKR (CpG4) were identified, respectively (P≤0.05). Our results indicate that cigarette smoking causes biochemical changes in the sperm DNA methylation in many regions and could adversely affect semen parameters.
DOI of the first publication: 10.1016/j.reprotox.2017.05.010
Link to this record: hdl:20.500.11880/27890
ISSN: 0890-6238
Date of registration: 25-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Jörn Walter
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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