Please use this identifier to cite or link to this item: doi:10.22028/D291-29430
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Title: Alterations in sperm DNA methylation patterns of oligospermic males
Author(s): Laqqan, Mohammed
Tierling, Sascha
Alkhaled, Yaser
LoPorto, Christina
Hammadeh, Mohamad Eid
Language: English
Title: Reproductive biology
Volume: 17
Issue: 4
Startpage: 396
Endpage: 400
Publisher/Platform: Elsevier Urban & Partner
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Aberrant in sperm DNA methylation patterns and histone modification play a role in male fecundity decline. This study was prepared to determine whether sperm DNA methylation at CpG dinucleotides is different in oligospermic males compared to proven fertile males and then to evaluate the correlation between the changes in sperm DNA methylation patterns and semen parameters of oligospermic males. A total of 165 males (64 proven fertile males "controls" and 101 oligospermic males "cases") were included in the study. Three CpG sites have the highest difference in methylation levels (cg23081194, cg25750688, and cg04807108) were underwent to further analysis using deep bisulfite sequencing in 125 samples (44 controls and 81 cases). The results of a validation study showed that variation in methylation levels was found in more than one CpG site: there was a significant alteration in methylation levels at all CpGs tested within the UBE2G2 and cg25750688 site related amplicon (p≤0.0001), and at eight CpGs (CpG1, CpG3, CpG6, CpG8, CpG11, CpG13, CpG14, and CpG15) within the cg04807108 site related amplicon (p≤0.0001) in cases compared to controls. Besides, a significant correlation was found between the changes in the methylation levels at different CpGs and semen parameters of case group. In conclusion, this study showed that these sites have a significant alteration in sperm DNA methylation levels in oligospermic males compared to proven fertile males, and these changes correlated with semen parameters.
DOI of the first publication: 10.1016/j.repbio.2017.10.007
Link to this record: hdl:20.500.11880/27888
http://dx.doi.org/10.22028/D291-29430
ISSN: 1642-431X
Date of registration: 25-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Collections:UniBib – Die Universitätsbibliographie

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