Please use this identifier to cite or link to this item: doi:10.22028/D291-29429
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Title: Blockage of the Epithelial-to-Mesenchymal Transition Is Required for Embryonic Stem Cell Derivation
Author(s): Totonchi, Mehdi
Hassani, Seyedeh-Nafiseh
Sharifi-Zarchi, Ali
Tapia, Natalia
Adachi, Kenjiro
Arand, Julia
Greber, Boris
Sabour, Davood
Araúzo-Bravo, Marcos J.
Walter, Jörn Erik
Pakzad, Mohammad
Gourabi, Hamid
Schöler, Hans R.
Baharvand, Hossein
Language: English
Title: Stem cell reports
Volume: 9
Issue: 4
Startpage: 1275
Endpage: 1290
Publisher/Platform: Elsevier
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Pluripotent cells emanate from the inner cell mass (ICM) of the blastocyst and when cultivated under optimal conditions immortalize as embryonic stem cells (ESCs). The fundamental mechanism underlying ESC derivation has, however, remained elusive. Recently, we have devised a highly efficient approach for establishing ESCs, through inhibition of the MEK and TGF-β pathways. This regimen provides a platform for dissecting the molecular mechanism of ESC derivation. Via temporal gene expression analysis, we reveal key genes involved in the ICM to ESC transition. We found that DNA methyltransferases play a pivotal role in efficient ESC generation. We further observed a tight correlation between ESCs and preimplantation epiblast cell-related genes and noticed that fundamental events such as epithelial-to-mesenchymal transition blockage play a key role in launching the ESC self-renewal program. Our study provides a time course transcriptional resource highlighting the dynamics of the gene regulatory network during the ICM to ESC transition.
DOI of the first publication: 10.1016/j.stemcr.2017.08.006
URL of the first publication:
Link to this record: hdl:20.500.11880/27887
ISSN: 2213-6711
Date of registration: 25-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Jörn Walter
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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