Please use this identifier to cite or link to this item: doi:10.22028/D291-28971
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Title: A glycerophospholipid-specific pocket in the RVFV class II fusion protein drives target membrane insertion
Author(s): Guardado-Calvo, P
Atkovska, K
Jeffers, S A
Grau, N
Backovic, M
Pérez-Vargas, J
de Boer, S M
Tortorici, M A
Pehau-Arnaudet, G
Lepault, J
England, P
Rottier, P J
Bosch, B J
Hub, Jochen Sebastian
Rey, F A
Language: English
Title: Science
Volume: 358
Issue: 6363
Startpage: 663
Endpage: 667
Publisher/Platform: American Association for the Advancement of Science (AAAS)
Year of Publication: 2017
Publikation type: Journal Article
Abstract: The Rift Valley fever virus (RVFV) is transmitted by infected mosquitoes, causing severe disease in humans and livestock across Africa. We determined the x-ray structure of the RVFV class II fusion protein Gc in its postfusion form and in complex with a glycerophospholipid (GPL) bound in a conserved cavity next to the fusion loop. Site-directed mutagenesis and molecular dynamics simulations further revealed a built-in motif allowing en bloc insertion of the fusion loop into membranes, making few nonpolar side-chain interactions with the aliphatic moiety and multiple polar interactions with lipid head groups upon membrane restructuring. The GPL head-group recognition pocket is conserved in the fusion proteins of other arthropod-borne viruses, such as Zika and chikungunya viruses, which have recently caused major epidemics worldwide.
DOI of the first publication: 10.1126/science.aal2712
Link to this record: hdl:20.500.11880/27792
ISSN: 1095-9203
Date of registration: 17-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Jochen Hub
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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