Bitte benutzen Sie diese Referenz, um auf diese Ressource zu verweisen:
Volltext verfügbar? / Dokumentlieferung
doi:10.22028/D291-28961
Titel: | Merging In-Solution X-ray and Neutron Scattering Data Allows Fine Structural Analysis of Membrane-Protein Detergent Complexes |
VerfasserIn: | Dias Mirandela, Gaëtan Tamburrino, Giulia Ivanović, Miloš T Strnad, Felix M Byron, Olwyn Rasmussen, Tim Hoskisson, Paul A Hub, Jochen Sebastian Zachariae, Ulrich Gabel, Frank Javelle, Arnaud |
Sprache: | Englisch |
Titel: | The journal of physical chemistry letters |
Bandnummer: | 9 |
Heft: | 14 |
Startseite: | 3910 |
Endseite: | 3914 |
Verlag/Plattform: | American Chemical Society (ACS) |
Erscheinungsjahr: | 2018 |
Dokumenttyp: | Journalartikel / Zeitschriftenartikel |
Abstract: | In-solution small-angle X-ray and neutron scattering (SAXS/SANS) have become popular methods to characterize the structure of membrane proteins, solubilized by either detergents or nanodiscs. SANS studies of protein-detergent complexes usually require deuterium-labeled proteins or detergents, which in turn often lead to problems in their expression or purification. Here, we report an approach whose novelty is the combined analysis of SAXS and SANS data from an unlabeled membrane protein complex in solution in two complementary ways. First, an explicit atomic analysis, including both protein and detergent molecules, using the program WAXSiS, which has been adapted to predict SANS data. Second, the use of MONSA which allows one to discriminate between detergent head- and tail-groups in an ab initio approach. Our approach is readily applicable to any detergent-solubilized protein and provides more detailed structural information on protein-detergent complexes from unlabeled samples than SAXS or SANS alone. |
DOI der Erstveröffentlichung: | 10.1021/acs.jpclett.8b01598 |
Link zu diesem Datensatz: | hdl:20.500.11880/27785 http://dx.doi.org/10.22028/D291-28961 |
ISSN: | 1948-7185 |
Datum des Eintrags: | 17-Sep-2019 |
Fakultät: | NT - Naturwissenschaftlich- Technische Fakultät |
Fachrichtung: | NT - Physik |
Professur: | NT - Prof. Dr. Jochen Hub |
Sammlung: | SciDok - Der Wissenschaftsserver der Universität des Saarlandes |
Dateien zu diesem Datensatz:
Es gibt keine Dateien zu dieser Ressource.
Alle Ressourcen in diesem Repository sind urheberrechtlich geschützt.