Please use this identifier to cite or link to this item: doi:10.22028/D291-28869
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Title: Maturation and cytokine pattern of human dendritic cells in response to different yeasts
Author(s): Bazan, Silvia Boschi
Walch-Rückheim, Barbara
Schmitt, Manfred J.
Breinig, Frank
Language: English
Title: Medical microbiology and immunology
Volume: 207
Issue: 1
Startpage: 75
Endpage: 81
Publisher/Platform: Springer
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Activated dendritic cells (DC) induce and polarize T-cell responses by expression of distinct maturation markers and cytokines. This study systematically investigated the capacity of different biotechnically relevant yeast species and strains including Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces lactis, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, and Candida glabrata to initiate maturation of human DC. As important prerequisite for T-cell activation, all yeasts were shown to effectively induce, though to a different extent, the expression of the activation marker CD83, the co-stimulatory molecules CD80, CD86, CD54, CD58, and CD40, as well as the antigen-presenting molecules MHCs I and II. Furthermore, yeast-activated DC secreted various cytokines including inflammatory TNF-α, IL-6, IL-8, and IL-1β or T-cell polarizing IL-12, IL-10, IL-23, and IL-27. Variability was observed in the expression of TNF-α, IL-6, IL-8, IL-1β, and IL-10 in response to the tested yeasts, whereas expression levels of IL-12, IL-23, and IL-27 were similar. Interestingly, maturation marker expression and cytokine secretion were not negatively affected after application of yeast mutants with altered cell wall mannoprotein structure (Δmnn11) or defective in protein N-glycosylation (Δost3), indicating that elongated cell wall mannoproteins at the outer yeast cell surface are not a prerequisite for the observed yeast-mediated DC maturation. Thus, our data provide a valuable basic knowledge for the future design of effective yeast-based delivery approaches.
DOI of the first publication: 10.1007/s00430-017-0528-8
Link to this record: hdl:20.500.11880/27760
ISSN: 0300-8584
Date of registration: 13-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Manfred Schmitt
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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