Please use this identifier to cite or link to this item: doi:10.22028/D291-28548
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Title: Genome mining reveals uncommon alkylpyrones as type III PKS products from myxobacteria
Author(s): Hug, Joachim Josef
Panter, Fabian
Krug, Daniel
Müller, Rolf
Language: English
Title: Journal of industrial microbiology and biotechnology : official journal of the Society for Industrial Microbiology
Volume: 46
Issue: 3-4
Startpage: 319
Endpage: 334
Publisher/Platform: Springer
Year of Publication: 2019
Publikation type: Journal Article
Abstract: Type III polyketide synthases (PKSs) are comparatively small homodimeric enzymes affording natural products with diverse structures and functions. While type III PKS biosynthetic pathways have been studied thoroughly in plants, their counterparts from bacteria and fungi are to date scarcely characterized. This gap is exemplified by myxobacteria from which no type III PKS-derived small molecule has previously been isolated. In this study, we conducted a genomic survey of myxobacterial type III PKSs and report the identification of uncommon alkylpyrones as the products of type III PKS biosynthesis from the myxobacterial model strain Myxococcus xanthus DK1622 through a self-resistance-guided screening approach focusing on genes encoding pentapetide repeat proteins, proficient to confer resistance to topoisomerase inhibitors. Using promoter-induced gene expression in the native host as well as heterologous expression of biosynthetic type III PKS genes, sufficient amounts of material could be obtained for structural elucidation and bioactivity testing, revealing potent topoisomerase activity in vitro.
DOI of the first publication: 10.1007/s10295-018-2105-6
URL of the first publication:
Link to this record: hdl:20.500.11880/27683
ISSN: 0169-4146
Date of registration: 6-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Pharmazie
Professorship: NT - Prof. Dr. Rolf Müller
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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