Please use this identifier to cite or link to this item: doi:10.22028/D291-28679
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Title: Transcriptome Kinetics of Saccharomyces cerevisiae in Response to Viral Killer Toxin K1
Author(s): Gier, Stefanie
Simon, Martin
Nordström, Karl
Khalifa, Salem
Schulz, Marcel H.
Schmitt, Manfred J.
Breinig, Frank
Language: English
Title: Frontiers in microbiology
Volume: 10
Startpage: 1
Endpage: 18
Publisher/Platform: Frontiers
Year of Publication: 2019
Publikation type: Journal Article
Abstract: The K1 A/B toxin secreted by virus-infected Saccharomyces cerevisiae strains kills sensitive cells via disturbance of cytoplasmic membrane functions. Despite decades of research, the mechanisms underlying K1 toxicity and immunity have not been elucidated yet. In a novel approach, this study aimed to characterize transcriptome changes in K1-treated sensitive yeast cells in a time-dependent manner. Global transcriptional profiling revealed substantial cellular adaptations in target cells resulting in 1,189 differentially expressed genes in total. Killer toxin K1 induced oxidative, cell wall and hyperosmotic stress responses as well as rapid down-regulation of transcription and translation. Essential pathways regulating energy metabolism were also significantly affected by the toxin. Remarkably, a futile cycle of the osmolytes trehalose and glycogen was identified probably representing a critical feature of K1 intoxication. In silico analysis suggested several transcription factors involved in toxin-triggered signal transduction. The identified transcriptome changes provide valuable hints to illuminate the still unknown molecular events leading to K1 toxicity and immunity implicating an evolutionarily conserved response at least initially counteracting ionophoric toxin action.
DOI of the first publication: 10.3389/fmicb.2019.01102
URL of the first publication: https://www.frontiersin.org/articles/10.3389/fmicb.2019.01102/full
Link to this record: hdl:20.500.11880/27670
http://dx.doi.org/10.22028/D291-28679
ISSN: 1664-302X
Date of registration: 6-Sep-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Biowissenschaften
Professorship: NT - Prof. Dr. Manfred Schmitt
Collections:Die Universitätsbibliographie

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