Please use this identifier to cite or link to this item: doi:10.22028/D291-28090
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Title: Comparative analysis of assays for detection of cell-mediated immunity toward cytomegalovirus and M. tuberculosis in samples from deceased organ donors
Author(s): Schmidt, T.
Schub, D.
Wolf, M.
Dirks, J.
Ritter, M.
Leyking, S.
Singh, M.
Zawada, A. M.
Blaes-Eise, A. B.
Samuel, U.
Sester, U.
Sester, M.
Sester, Martina
Title: American Journal of Transplantation
Volume: 14
Issue: 9
Startseite: 2159
Endseite: 2167
Publisher/Platform: Wiley-Blackwell
Year of Publication: 2014
Publikation type: Journal Article
Abstract: Cell-mediated immunity assays could be valuable for risk assessment of organ donors, but no data exist on their feasibility in deceased donors. In this study, 105 deceased donors (52.3 +/- 16.9 years) were screened at the time of organ procurement. Pathogen-specific stimulation was performed using a cytomegalovirus (CMV) lysate, tuberculin (purified protein derivative [PPD]) and soluble Mycobacterium tuberculosis-specific ESAT-6/CFP-10 proteins in combination with an in-house fluorescence-activated cell sorting (FACS) assay or commercial assay formats (QuantiFERON-CMV/TB for ELISA, T-SPOT.TB for ELISPOT). CMV-IgG antibody titers were determined as gold standard for CMV infection; 51.4% of samples were CMV seropositive. Indeterminate results were observed in 47.6% of ELISA, 12.5% of FACS and 0% of ELISPOT assays. Agreement with serology was highest for FACS (95.6%, kappa = 0.91), followed by ELISPOT (84.0%, kappa = 0.68) and ELISA (80.0%, kappa = 0.60). Agreement between ELISA and serology increased if the CMV lysate was used as stimulus (96.7%, kappa = 0.92). Among the T cell assays, agreement between ELISPOT and FACS was highest (kappa = 0.70). PPD-positive results among valid samples differed between assays (26.5% for ELISA, 23.1% for FACS and 50.5% for ELISPOT); 2.0% were QuantiFERON-TB positive, 3.3% were ESAT-6/CFP-10-positive in FACS and 13.4% were positive in the T-SPOT.TB assay. In conclusion, cellular immunity may be analyzed from samples of deceased donors, although the assays differ in the rate of positivity and indeterminate results.
DOI of the first publication: 10.1111/ajt.12787
URL of the first publication: https://www.ncbi.nlm.nih.gov/pubmed/25040687
Link to this record: hdl:20.500.11880/27653
http://dx.doi.org/10.22028/D291-28090
ISSN: 1600-6143 (Electronic)1600-6135 (Linking)
Date of registration: 10-Aug-2019
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Collections:UniBib – Die Universitätsbibliographie

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