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|Title:||Rapid reconstitution of CMV-specific T-cells after stem-cell transplantation|
Gärtner, Barbara C.
|Title:||European Journal of Haematology|
|Publisher/Platform:||Wiley-Blackwell - STM|
|Year of Publication:||2018|
|Publikation type:||Journal Article|
|Abstract:||OBJECTIVE: As reconstitution of virus-specific T-cells is critical to control cytomegalovirus (CMV)-viremia following stem-cell transplantation (SCT), we characterized the dynamics in CMV-specific T-cell reconstitution after SCT. METHODS: Cytomegalovirus-specific T-cells from 51 SCT-recipients were prospectively quantified and phenotypically characterised by intracellular cytokine-staining after specific stimulation and HLA class-I-specific pentamers using flow cytometry. RESULTS: Cytomegalovirus-specific CD4 T-cells reconstituted after a median of 2.3 (IQR, 2.0-3.0) weeks following autografting, and 4.0 (IQR, 3.0-5.6) weeks after allografting, with CMV-specific T-cells originating from donors and/or recipients. The time for reconstitution of CMV-specific CD4 and CD8 T-cells did not differ (P = .58). Factors delaying the time to initial reconstitution of CMV-specific CD4 T-cells included a negative recipient serostatus (P = .016) and CMV-viremia (P = .026). Percentages of CMV-specific CD4 T-cells significantly increased over time and reached a plateau after 90 days (P = .043). Relative CMV-specific CD4 T-cell levels remained higher in long-term transplant recipients compared with those in controls (P < .0001). However, due to persisting lymphopenia, absolute numbers of CMV-specific T-cells were similar as in controls. CONCLUSION: Cytomegalovirus-specific T-cells rapidly reconstitute after SCT and their percentages remain high in the long term. In the face of persistent lymphopenia, this results in similar absolute numbers of CMV-specific T-cells as in controls to ensure sufficient pathogen control.|
|DOI of the first publication:||10.1111/ejh.13077|
|URL of the first publication:||https://www.ncbi.nlm.nih.gov/pubmed/29652096|
|Link to this record:||hdl:20.500.11880/27652|
|Date of registration:||10-Aug-2019|
|Third-party funds sponsorship:||José Carreras Leukämie Stiftung|
|Faculty:||M - Medizinische Fakultät|
|Department:||M - Infektionsmedizin|
|Professorship:||M - Prof. Dr. Martina Sester|
|Collections:||UniBib – Die Universitätsbibliographie|
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