Please use this identifier to cite or link to this item: doi:10.22028/D291-28067
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Title: Rapid reconstitution of CMV-specific T-cells after stem-cell transplantation
Author(s): Widmann, Thomas
Sester, Urban
Gärtner, Barbara C.
Schubert, Jörg
Pfreundschuh, Michael
Sester, Martina
Language: English
Title: European Journal of Haematology
Volume: 101
Issue: 1
Startpage: 38
Endpage: 47
Publisher/Platform: Wiley-Blackwell - STM
Year of Publication: 2018
Publikation type: Journal Article
Abstract: OBJECTIVE: As reconstitution of virus-specific T-cells is critical to control cytomegalovirus (CMV)-viremia following stem-cell transplantation (SCT), we characterized the dynamics in CMV-specific T-cell reconstitution after SCT. METHODS: Cytomegalovirus-specific T-cells from 51 SCT-recipients were prospectively quantified and phenotypically characterised by intracellular cytokine-staining after specific stimulation and HLA class-I-specific pentamers using flow cytometry. RESULTS: Cytomegalovirus-specific CD4 T-cells reconstituted after a median of 2.3 (IQR, 2.0-3.0) weeks following autografting, and 4.0 (IQR, 3.0-5.6) weeks after allografting, with CMV-specific T-cells originating from donors and/or recipients. The time for reconstitution of CMV-specific CD4 and CD8 T-cells did not differ (P = .58). Factors delaying the time to initial reconstitution of CMV-specific CD4 T-cells included a negative recipient serostatus (P = .016) and CMV-viremia (P = .026). Percentages of CMV-specific CD4 T-cells significantly increased over time and reached a plateau after 90 days (P = .043). Relative CMV-specific CD4 T-cell levels remained higher in long-term transplant recipients compared with those in controls (P < .0001). However, due to persisting lymphopenia, absolute numbers of CMV-specific T-cells were similar as in controls. CONCLUSION: Cytomegalovirus-specific T-cells rapidly reconstitute after SCT and their percentages remain high in the long term. In the face of persistent lymphopenia, this results in similar absolute numbers of CMV-specific T-cells as in controls to ensure sufficient pathogen control.
DOI of the first publication: 10.1111/ejh.13077
URL of the first publication:
Link to this record: hdl:20.500.11880/27652
ISSN: 1600-0609
Date of registration: 10-Aug-2019
Third-party funds sponsorship: José Carreras Leukämie Stiftung
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Professorship: M - Prof. Dr. Martina Sester
Collections:Die Universitätsbibliographie

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