Please use this identifier to cite or link to this item: doi:10.22028/D291-28071
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Title: Evaluation of antigen specific interleukin-1β as a biomarker to detect cattle infected with Mycobacterium bovis
Author(s): Elnaggar, Mahmoud M.
Abdellrazeq, Gaber S.
Elsisy, Alaa
Mahmoud, Asmaa H.
Shyboub, Abdelrazeq
Sester, Martina
Khaliel, Samy A.
Singh, Mahavir
Torky, Helmy A.
Davis, William C.
Language: English
Title: Tuberculosis
Volume: 105
Startpage: 53
Endpage: 59
Publisher/Platform: Elsevier
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Bovine tuberculosis (bTB) is a major world-wide health problem that has been difficult to control, due to the lack of an effective vaccine and limited ability of the tuberculin skin test (TST) and the ancillary whole blood interferon-gamma (IFN-γ) release assay (IGRA) to detect all infected animals. A 6 h cytokine flow cytometric IFN-γ (CFC) assay was developed in effort to overcome these limitations and expand methods for studying the mechanisms of bTB immunopathogenesis. The present study was conducted to evaluate IL-1β as a biomarker to use in conjunction with the IFN-γ CFC assay to improve the diagnostic accuracy for bTB. Three animal groups with predefined Mbv infection status were used for analysis of IL-1β in plasma from whole blood cultures stimulated with ESAT-6/CFP-10 for 20-24 h. Parallel stimulations were performed for enumeration of IFN-γ producing T cells. Data analysis showed that Mbv infected animals have a higher frequency of IFN-γ producing CD4+ T cells and plasma IL-1β than animals exposed to non-tuberculous mycobacteria (NTM) or uninfected control animals, with a significant correlation between the two readouts, thus allowing differentiation between the three animal groups. IL-1β has the potential to serve as an additional biomarker for detecting cattle infected with Mbv.
DOI of the first publication: 10.1016/j.tube.2017.04.009
URL of the first publication: https://www.ncbi.nlm.nih.gov/pubmed/28610788
Link to this record: hdl:20.500.11880/27528
http://dx.doi.org/10.22028/D291-28071
ISSN: 1873-281X
1472-9792
Date of registration: 13-Jul-2019
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Collections:UniBib – Die Universitätsbibliographie

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