Please use this identifier to cite or link to this item: doi:10.22028/D291-28073
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Title: Donor-specific alloreactive T cells can be quantified from whole blood, and may predict cellular rejection after renal transplantation
Author(s): Fischer, Michaela
Leyking, Sarah
Schäfer, Marco
Elsäßer, Julia
Janssen, Martin
Mihm, Janine
van Bentum, Kai
Fliser, Danilo
Sester, Martina
Sester, Urban
Language: English
Title: European Journal of Immunology
Volume: 47
Issue: 7
Startpage: 1220
Endpage: 1231
Publisher/Platform: Wiley-Blackwell - STM
Year of Publication: 2017
Publikation type: Journal Article
Abstract: Preformed cellular alloreactivity can exist prior to transplantation and may contribute to rejection. Here, we used a rapid flow-cytometric whole-blood assay to characterize the extent of alloreactive T cells among 1491 stimulatory reactions from 61 renal transplant candidates and 75 controls. The role of preformed donor-specific alloreactive T cells in cellular rejection was prospectively analyzed in 21 renal transplant recipients. Alloreactive CD8+ T cells were more frequent than respective CD4+ T cells, and these levels were stable over time. CD8+ T cells were effector-memory T cells largely negative for expression of CD27, CD62L, and CCR7, and were susceptible to steroid and calcineurin inhibitor inhibition. Alloreactivity was more frequent in samples with higher number of HLA mismatches. Moreover, the percentage of individuals with alloreactive T cells was higher in transplant candidates than in controls. Among transplant candidates, 5/61 exhibited alloreactive CD8+ T cells against most stimulators, 23/61 toward a limited number of stimulators, and 33/61 did not show any alloreactivity. Among 21 renal transplant recipients followed prospectively, one had donor-specific preformed T-cell alloreactivity. She was the only patient who developed cellular rejection posttransplantation. In conclusion, donor-specific alloreactive T cells may be rapidly quantified from whole blood, and may predict cellular rejection after transplantation.
DOI of the first publication: 10.1002/eji.201646826
URL of the first publication:
Link to this record: hdl:20.500.11880/27527
ISSN: 1521-4141
Date of registration: 13-Jul-2019
Faculty: M - Medizinische Fakultät
Department: M - Infektionsmedizin
Professorship: M - Prof. Dr. Martina Sester
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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