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|Title:||CMV-specific T-cells and CD27-CD28-CD4+ T-cells for assignment of cytomegalovirus (CMV) status in adults awaiting organ transplant|
|Author(s):||Burton, Catherine E.|
Robinson, Joan L.
Eurich, Dean T.
Preiksaitis, Jutta K.
|Title:||Journal of Clinical Virology|
|Year of Publication:||2019|
|Publikation type:||Journal Article|
|Abstract:||BACKGROUND/OBJECTIVES: Determination of Cytomegalovirus (CMV) status in solid organ transplant (SOT) candidates is essential to stratify risk of post-transplant CMV disease. Passive transfusion-acquired antibodies can make serologic determination of CMV status unreliable. We evaluated 3 assays, not affected by passive antibodies (PA), in assignment of CMV status: quantification of CMV-specific CD4 + T-cells (CMV-TC) and exhausted CD27-CD28- CD4 + T-cells, and detection of CMV DNA with Nucleic Acid Amplification Testing (NAAT). STUDY DESIGN: We enrolled 50 adults awaiting SOT and 50 immunocompetent age-matched controls, and collected a throat swab, urine, saliva and blood sample on each. Using flow cytometry CD4 + T-cells were phenotypically analyzed for expression of CD27 and CD28 and CMV-specific CD4 + T-cells were identified by CD69 expression and intracellular IFN-γ quantification after stimulation with CMV-antigen lysate. CMV NAAT was performed on all specimens using real-time PCR. CMV serology (CMV IgG) was determined by enzyme immunoassay. Subjects were considered to have potential PA if they received blood products within 2 months of collection. RESULTS: The CMV-TC assay discriminated between CMV-seropositive and seronegative SOT candidates without PA well (sensitivity 79%, specificity 93%) while the CD27-CD28-CD4 + T-cell assay had good sensitivity (86%) but specificity of 74%. Detection of CMV DNA was uncommon in CMV-seropositive SOT candidates (2/21). CONCLUSIONS: Given its high specificity, the CMV-TC assay is valuable in confirming true-positive CMV status in seropositive SOT candidates with PA, while use of CD27-CD28-CD4 + T-cell analysis is limited by moderate specificity. Detection of CMV DNA is of limited value in assignment of CMV status in adults.|
|DOI of the first publication:||10.1016/j.jcv.2019.03.014|
|URL of the first publication:||https://www.ncbi.nlm.nih.gov/pubmed/30959325|
|Link to this record:||hdl:20.500.11880/27489|
|Date of registration:||13-Jul-2019|
|Third-party funds sponsorship:||Institutional funds|
|Faculty:||M - Medizinische Fakultät|
|Department:||M - Infektionsmedizin|
|Professorship:||M - Prof. Dr. Martina Sester|
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