Please use this identifier to cite or link to this item: doi:10.22028/D291-28015
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Title: Determination of the rheological properties of Matrigel for optimum seeding conditions in microfluidic cell cultures
Author(s): Kane, Khalid I. W.
Moreno, E. Lucumi
Lehr, Claudius Moritz
Hachi, Siham
Dannert, Rick
Sanctuary, Roland
Fleming, Ronan M. T.
Baller, Jörg
Wagner, Christian
Title: AIP Advances
Volume: 8
Issue: 12
Publisher/Platform: American Institute of Physics
Year of Publication: 2018
Publikation type: Journal Article
Abstract: Hydrogels are increasingly used as a surrogate extracellular matrix in three-dimensional cell culture systems, including microfluidic cell culture. Matrigel is a hydrogel of natural origin widely used in cell culture, particularly in the culture of stem cell-derived cell lines. The use of Matrigel as a surrogate extracellular matrix in microfluidic systems is challenging due to its biochemical, biophysical, and biomechanical properties. Therefore, understanding and characterising these properties is a prerequisite for optimal use of Matrigel in microfluidic systems. We used rheological measurements and particle image velocimetry to characterise the fluid flow dynamics of liquefied Matrigel during loading into a three-dimensional microfluidic cell culture device. Using fluorescence microscopy and fluorescent beads for particle image velocimetry measurements (velocity profiles) in combination with classical rheological measurements of Matrigel (viscosity versus shear rate), we characterised the shear rates experienced by cells in a microfluidic device for three-dimensional cell culture. This study provides a better understanding of the mechanical stress experienced by cells, during seeding of a mixture of hydrogel and cells, into three-dimensional microfluidic cell culture devices.
DOI of the first publication: 10.1063/1.5067382
URL of the first publication: https://aip.scitation.org/doi/10.1063/1.5067382
Link to this record: hdl:20.500.11880/27450
http://dx.doi.org/10.22028/D291-28015
ISSN: 2158-3226
Date of registration: 11-Jun-2019
Faculty: NT - Naturwissenschaftlich- Technische Fakultät
Department: NT - Physik
Professorship: NT - Prof. Dr. Christian Wagner
Collections:UniBib – Die Universitätsbibliographie

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