Please use this identifier to cite or link to this item: doi:10.22028/D291-24788
Title: Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages
Author(s): Hoppstädter, Jessica
Diesel, Britta
Zarbock, Robert
Breinig, Tanja
Monz, Dominik
Koch, Marcus
Meyerhans, Andreas
Gortner, Ludwig
Lehr, Claus-Michael
Huwer, Hanno
Kiemer, Alexandra K.
Language: English
Year of Publication: 2010
OPUS Source: Respiratory research. - 11. 2010, S. 124-138
SWD key words: Makrophagen-Aktivierungsfaktor
DDC notations: 620 Engineering and machine engineering
Publikation type: Journal Article
Abstract: BACKGROUND: Investigations on pulmonary macrophages (MΦ) mostly focus on alveolar MΦ (AM) as a well-defined cell population. Characteristics of MΦ in the interstitium, referred to as lung interstitial MΦ (IM), are rather ill-defined. In this study we therefore aimed to elucidate differences between AM and IM obtained from human lung tissue. METHODS: Human AM and IM were isolated from human non-tumor lung tissue from patients undergoing lung resection. Cell morphology was visualized using either light, electron or confocal microscopy. Phagocytic activity was analyzed by flow cytometry as well as confocal microscopy. Surface marker expression was measured by flow cytometry. Toll-like receptor (TLR) expression patterns as well as cytokine expression upon TLR4 or TLR9 stimulation were assessed by real time RT-PCR and cytokine protein production was measured using a fluorescent bead-based immunoassay. RESULTS: IM were found to be smaller and morphologically more heterogeneous than AM, whereas phagocytic activity was similar in both cell types. HLA-DR expression was markedly higher in IM compared to AM. Although analysis of TLR expression profiles revealed no differences between the two cell populations, AM and IM clearly varied in cell reaction upon activation. Both MΦ populations were markedly activated by LPS as well as DNA isolated from attenuated mycobacterial strains (M. bovis H37Ra and BCG). Whereas AM expressed higher amounts of inflammatory cytokines upon activation, IM were more efficient in producing immunoregulatory cytokines, such as IL10, IL1ra, and IL6.CONCLUSION: AM appear to be more effective as a non-specific first line of defence against inhaled pathogens, whereas IM show a more pronounced regulatory function. These dissimilarities should be taken into consideration in future studies on the role of human lung MΦ in the inflammatory response.
Link to this record: urn:nbn:de:bsz:291-scidok-33939
Date of registration: 15-Mar-2011
Faculty: SE - Sonstige Einrichtungen
Department: SE - INM Leibniz-Institut für Neue Materialien
NT - Pharmazie
SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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