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Titel: Stabilisation by freeze-drying of cationically modified silica nanoparticles for gene delivery
Verfasser: Sameti, M.
Bohr, G.
Ravi Kumar, M. N. V.
Kneuer, Carsten
Bakowsky, Udo
Nacken, Manfred
Schmidt, Helmut K.
Lehr, C.-M.
Sprache: Englisch
Erscheinungsjahr: 2003
Quelle: International journal of pharmaceutics. - 266. 2003, 1/2, S. 51-60
SWD-Schlagwörter: Siliciumdioxid
Nanopartikel
Aggregation
Gefriertrocknung
Trehalose
Glycerin
DDC-Sachgruppe: 500 Naturwissenschaften
Dokumentart : Journalartikel
Kurzfassung: Core shell silica particles with a hydrodynamic diameter of 28nm, an IEP of 7.1 and a zeta potential of +35mV at pH 4.0 were synthesised. The role of freeze-drying for the conservation of zwitterionic nanoparticles and the usefulness of different lyoprotective agents (LPA) for the minimisation of particle aggregation were studied. The activity of the nanoparticles was measured as DNA-binding capacity and transfection efficiency in Cos-1 cells before and after lyophilisation. It was found that massive aggregation occurred in the absence of LPA. Of the various LPAs screened in the present investigations, trehalose and glycerol were found to be well suited for conservation of cationically modified silica nanoparticles with simultaneous preservation of their DNA-binding and transfection activity in Cos-1 cells.
Link zu diesem Datensatz: urn:nbn:de:bsz:291-scidok-30655
hdl:20.500.11880/24797
http://dx.doi.org/10.22028/D291-24741
SciDok-Publikation: 15-Feb-2011
Fakultät: Sonstige Einrichtungen
Fachrichtung: SE - INM Leibniz-Institut für Neue Materialien
Fakultät / Institution:INM
SE - Sonstige Einrichtungen

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