Please use this identifier to cite or link to this item: doi:10.22028/D291-24741
Title: Stabilisation by freeze-drying of cationically modified silica nanoparticles for gene delivery
Author(s): Sameti, M.
Bohr, G.
Ravi Kumar, M. N. V.
Kneuer, Carsten
Bakowsky, Udo
Nacken, Manfred
Schmidt, Helmut K.
Lehr, C.-M.
Language: English
Year of Publication: 2003
OPUS Source: International journal of pharmaceutics. - 266. 2003, 1/2, S. 51-60
SWD key words: Siliciumdioxid
DDC notations: 500 Science
Publikation type: Journal Article
Abstract: Core shell silica particles with a hydrodynamic diameter of 28nm, an IEP of 7.1 and a zeta potential of +35mV at pH 4.0 were synthesised. The role of freeze-drying for the conservation of zwitterionic nanoparticles and the usefulness of different lyoprotective agents (LPA) for the minimisation of particle aggregation were studied. The activity of the nanoparticles was measured as DNA-binding capacity and transfection efficiency in Cos-1 cells before and after lyophilisation. It was found that massive aggregation occurred in the absence of LPA. Of the various LPAs screened in the present investigations, trehalose and glycerol were found to be well suited for conservation of cationically modified silica nanoparticles with simultaneous preservation of their DNA-binding and transfection activity in Cos-1 cells.
Link to this record: urn:nbn:de:bsz:291-scidok-30655
Date of registration: 15-Feb-2011
Faculty: SE - Sonstige Einrichtungen
Department: SE - INM Leibniz-Institut für Neue Materialien
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