Please use this identifier to cite or link to this item: doi:10.22028/D291-24727
Title: Modified silica particles for gene delivery
Author(s): Csögör, Zsuzsa
Nacken, Manfred
Sameti, Mohammad
Lehr, Claus-Michael
Schmidt, Helmut K.
Language: English
Year of Publication: 2003
OPUS Source: Materials science & engineering / C. - 23. 2003, 1/2, S. 93-97
SWD key words: Siliciumdioxid
DDC notations: 620 Engineering and machine engineering
Publikation type: Journal Article
Abstract: In somatic gene therapy, new concepts for the transfer of DNA into specific cell nuclei are of interest. Inorganic nanoparticles have an interesting potential as DNA carrier system due to the possibility to tailor their surface reactivity and the electrical surface potential (zeta potential) that can be obtained by the surface modification. SiO2 nanoparticles have been chosen for reasons of low toxicity. In order to obtain positively charged nanoparticles, basic surface groupings have been selected. Different types of alkoxysilanes and amines have been tested for surface modification Of SiO2 nanoparticles. The zeta potential at pH = 7.4 could be varied from - 38.8 mV (unmodified 10 nm particles) to + 20 mV (gamma-aminopropyl-triethoxysilane) and to + 49.8 mV (glycidoxypropyltrimethoxysilane and ethylendiamine). DNA could be completely immobilized at the nanoparticle surface and nanoparticle/DNA ratios between 2 and 15 (w/w) were obtained. The interaction of DNA with the particle surface correlates with an increasing number of modifier molecules on the particle surface.
Link to this record: urn:nbn:de:bsz:291-scidok-30511
Date of registration: 2-Dec-2010
Faculty: SE - Sonstige Einrichtungen
Department: SE - INM Leibniz-Institut für Neue Materialien
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