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Titel: Modified silica particles for gene delivery
Verfasser: Csögör, Zsuzsa
Nacken, Manfred
Sameti, Mohammad
Lehr, Claus-Michael
Schmidt, Helmut K.
Sprache: Englisch
Erscheinungsjahr: 2003
Quelle: Materials science & engineering / C. - 23. 2003, 1/2, S. 93-97
SWD-Schlagwörter: Siliciumdioxid
Nanopartikel
Oberfläche
Modifizierung
Aminosilane
DNS
Gentransfer
DDC-Sachgruppe: 620 Ingenieurwissenschaften und Maschinenbau
Dokumentart : Journalartikel
Kurzfassung: In somatic gene therapy, new concepts for the transfer of DNA into specific cell nuclei are of interest. Inorganic nanoparticles have an interesting potential as DNA carrier system due to the possibility to tailor their surface reactivity and the electrical surface potential (zeta potential) that can be obtained by the surface modification. SiO2 nanoparticles have been chosen for reasons of low toxicity. In order to obtain positively charged nanoparticles, basic surface groupings have been selected. Different types of alkoxysilanes and amines have been tested for surface modification Of SiO2 nanoparticles. The zeta potential at pH = 7.4 could be varied from - 38.8 mV (unmodified 10 nm particles) to + 20 mV (gamma-aminopropyl-triethoxysilane) and to + 49.8 mV (glycidoxypropyltrimethoxysilane and ethylendiamine). DNA could be completely immobilized at the nanoparticle surface and nanoparticle/DNA ratios between 2 and 15 (w/w) were obtained. The interaction of DNA with the particle surface correlates with an increasing number of modifier molecules on the particle surface.
Link zu diesem Datensatz: urn:nbn:de:bsz:291-scidok-30511
hdl:20.500.11880/24783
http://dx.doi.org/10.22028/D291-24727
SciDok-Publikation: 2-Dez-2010
Fakultät: Sonstige Einrichtungen
Fachrichtung: SE - INM Leibniz-Institut für Neue Materialien
Fakultät / Institution:INM
SE - Sonstige Einrichtungen

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