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Titel: Silica nanoparticles modified with aminosilanes as carriers for plasmid DNA
Verfasser: Lehr, Claus-Michael
Kneuer, Carsten
Sameti, Mohammad
Haltner, Eleonore G.
Schiestel, Thomas
Schirra, Hermann
Schmidt, Helmut K.
Sprache: Englisch
Erscheinungsjahr: 2000
Quelle: International journal of pharmaceutics. - 196. 2000, 2, S. 257-261
SWD-Schlagwörter: Siliciumdioxid
Nanopartikel
Aminosilane
DNS
Carrier
Plasmid
Absorption
DDC-Sachgruppe: 540 Chemie
Dokumentart : Journalartikel
Kurzfassung: We synthesised silica nanoparticles (SiNP) with covalently linked cationic surface modifications and demonstrated their ability to electrostatically bind, condense and protect plasmid DNA. These particles might be utilised as DNA carriers for gene delivery. All nanoparticles were sized between 10 and 100 nm and displayed surface charge potentials from +7 to +31mV at pH 7.4. They were produced by modification of commercially available (IPAST) or in-house synthesised silica particles with either N-(2-aminoethyl)-3-aminopropyltrimethoxysilane or N-(6-aminohexyl)-3-aminopropyltrimethoxysilane. All particles formed complexes with pCMVbeta plasmid DNA as evidenced by ratio dependend retardation of DNA in the agarosegel and co-sedimentation of soluble DNA with nanoparticles. High salt and alkaline pH did inhibit complex formation. Absorption onto the particles also decreased the hydrodynamic dimensions of plasmid DNA as shown by photon correlation spectroscopy. Complexes formed in water at a w/w ratio of Si26H:DNA (pCMVbeta) of 300 were smallest with a mean hydrodynamic diameter of 83 nm. For effective condensation a w/w ratio of Si26H:DNA of 30 was sufficient. Further, the absorbed DNA was protected from enzymatic degradation by DNase I.
Link zu diesem Datensatz: urn:nbn:de:bsz:291-scidok-29828
hdl:20.500.11880/24717
http://dx.doi.org/10.22028/D291-24661
SciDok-Publikation: 6-Sep-2010
Fakultät: Sonstige Einrichtungen
Fachrichtung: SE - INM Leibniz-Institut für Neue Materialien
Fakultät / Institution:INM
SE - Sonstige Einrichtungen

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