Please use this identifier to cite or link to this item: doi:10.22028/D291-21738
Title: CK2 kinase activity but not its binding to CK2 promoter regions is implicated in the regulation of CK2α and CK2β gene expressions
Author(s): Lupp, Sarah
Gumhold, Catalina
Ampofo, Emmanuel
Montenarh, Mathias
Rother, Karen
Language: English
Year of Publication: 2013
OPUS Source: Molecular and cellular biochemistry. - 2013, DOI: 10.1007/s11010-013-1782-8 [Elektronische Ressource]
SWD key words: Proteinkinase CK2
Transkription <Genetik>
Free key words: Transkriptionsregulation
protein kinase CK2
gene expression
transcriptional regulation
DNA binding
DDC notations: 500 Science
Publikation type: Report
Abstract: Protein kinase CK2, a ubiquitous serine/threonine kinase in control of a variety of crucial cellular functions, is composed of catalytic a- and a0-subunits and non-catalytic b-subunits which form holoenzymes such as CK2(ab)2, CK2aa'b2, or CK2(a'b)2. In addition, there is sample evidence for the occurrence of the individual subunits beside the holoenzyme. While the CK2 subunits are well analyzed on the protein level, only little is known about the regulation of their transcription. The existence of multiple forms of CK2 subunits raised the question about a mutual regulation of their expression. Here we defined two 50-upstream regions of the CK2alpha and the CK2beta genes, respectively, as sequences with promoter activities. We found that CK2alpah and CK2alpha' stimulated the expression of the reporter constructs whereas, CK2beta was inactive. Using chromatin immunoprecipitation assays, we were unable to detect binding of endogenous CK2 subunits to these promoter sequences in vivo. However, it turned out that inhibition of the kinase activity of CK2 attenuated the promoter activity indicating that CK2alpha and CK2alpha' might regulate their gene expression indirectly by phosphorylation reactions. Thus, we have shown here (i) that under normal physiological conditions CK2 does not bind to CK2 promoter regions and (ii) that the CK2 kinase activity is implicated in the regulation of its own expression.
Link to this record: urn:nbn:de:bsz:291-scidok-55052
Date of registration: 27-Sep-2013
Faculty: M - Medizinische Fakultät
Department: M - Medizinische Biochemie und Molekularbiologie
ZE - Zentrum für Human- und Molekularbiologie (ZHMB)
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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