Please use this identifier to cite or link to this item: doi:10.22028/D291-21016
Title: v-SNARE actions during Ca²⁺-triggered exocytosis
Author(s): Kesavan, Jaideep
Borisovska, Maria
Bruns, Dieter
Language: English
Year of Publication: 2007
OPUS Source: Cell. - 131. 2007, 2, S. 351-363
Free key words: SNARE
Ca2+ increase
DDC notations: 570 Life sciences, biology
Publikation type: Journal Article
Abstract: Assembly of SNARE proteins between opposing membranes mediates fusion of synthetic lipo-somes, but it is unknown whether SNAREs act during exocytosis at the moment of Ca²⁺ increase, providing the molecular force for fusion of secretory vesicles. Here, weshowthatexecution of pre- and postfusional Steps during chromaffin granule exocytosis depends crucially on a short molecular distance between the complex-forming SNARE motif and the transmembrane anchor of the vesicular SNARE protein synaptobrevin II. Extending the juxtamembrane region of synaptobrevin by insertion of flexible "linkers" reduces priming of granules, delays initiation of exocytosis upon stepwise elevation of intracellular calcium, attenuates fluctuations of early fusion pores, and slows rapid expansion of the pore in a linker-length dependent fashion. These observations provide evidence that v-SNARE proteins drive Ca²⁺-triggered membrane fusion at millisecond time scale and support a model wherein continuous molecular pulling by SNAREs guides the vesicle throughout the consecutive stages of exocytosis.
Link to this record: urn:nbn:de:bsz:291-scidok-16419
Date of registration: 21-Jul-2008
Faculty: M - Medizinische Fakultät
Department: M - Physiologie
Collections:SciDok - Der Wissenschaftsserver der Universität des Saarlandes

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