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Titel: Local stimulation of articular cartilage repair by transplantation of encapsulated chondrocytes overexpressing human fibroblast growth factor 2 (FGF-2) in vivo
Verfasser: Kaul, Gunter
Cucchiarini, Magali
Arntzen, David
Zurakowski, David
Menger, Michael D.
Kohn, Dieter
Trippel, Stephen B.
Madry, Henning
Sprache: Deutsch
Erscheinungsjahr: 2006
Quelle: The journal of gene medicine. - 8. 2006, 1, 100-111
DDC-Sachgruppe: 610 Medizin, Gesundheit
Dokumentart : Journalartikel
Kurzfassung: Background Defects of articular cartilage are an unsolved problem in orthopaedics. In the present study, we tested the hypothesis that gene transfer of human fibroblast growth factor 2 (FGF-2) via transplantation of encapsulated genetically modified articular chondrocytes stimulates chondrogenesis in cartilage defects in vivo. Methods Lapine articular chondrocytes overexpressing a lacZ or a human FGF-2 gene sequence were encapsulated in alginate and further characterized. The resulting lacZ or FGF-2 spheres were applied to cartilage defects in the knee joints of rabbits. In vivo, cartilage repair was assessed qualitatively and quantitatively at 3 and 14 weeks after implantation. Results In vitro, bioactive FGF-2 was secreted, leading to a significant increase in the cell numbers in FGF-2 spheres. In vivo, FGF-2 continued to be expressed for at least 3 weeks without leading to differences in FGF-2 concentrations in the synovial fluid between treatment groups. Histological analysis revealed no adverse pathologic effects on the synovial membrane at any time point. FGF-2 gene transfer enhanced type II collagen expression and individual parameters of chondrogenesis, such as the cell morphology and architecture of the new tissue. Overall articular cartilage repair was significantly improved at both time points in vivo. Conclusions The data suggest that localized overexpression of FGF-2 enhances the repair of cartilage defects via stimulation of chondrogenesis, without adverse effects on the synovial membrane. These results may lead to the development of safe gene-based therapies for human articular cartilage defects.
Link zu diesem Datensatz: urn:nbn:de:bsz:291-scidok-14971
hdl:20.500.11880/21034
http://dx.doi.org/10.22028/D291-20978
SciDok-Publikation: 24-Apr-2008
Fakultät: Fakultät 2 - Medizin
Fachrichtung: M - Orthopädie
Fakultät / Institution:M - Medizinische Fakultät

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